How to grow Oyster Mushrooms on straw and substrates (2024)

Please note: Blue Dolphin™ and Polar White™ Oyster spawn are log specific strains, and are not recommended in substrate applications. King Oyster and Black Pearl Oyster are only suitable for production on a sterilized sawdust substrate.

Growing Oyster mushrooms on pasteurized straw can be one of the most rewarding production ventures for mushroom growers. With the current emphasis on local foods and local farm markets there is hardly another crop that is so well suited. Oyster mushrooms have a relatively short shelf life and are a bit fragile, so they are best hand carried and sold within a week. When fresh, they are some of the most stunning and colorful produce available, perfect for the market gardener.

Oyster mushrooms can grow on a variety of things including wood logs, cottonseed hulls, corn cobs or office paper, but in North America, cereal straws are the substrate of choice (wheat and oat straw do particularly well). Equipment is fairly simple and outstanding results are obtained relatively quickly. It can be fraught with its own problems as scale-up commences, however, so be prepared to research and proceed carefully as you prepare to increase production.

Straw must be moisturized to host the growth of the mushroom mycelium and treated with hydrated lime or heat. Wood logs do not need such treatment as they are already moist and have a protective sleeve (the bark) slowing moisture loss and providing a semi-sterile environment. In addition, living green trees cut for Oyster cultivation have an active immune system that holds back contaminants.

The Basic Steps for Growing Oysters on Straw

1. Prepare the substrate
Straw must be moisturized to promote the growth of the mushroom mycelium and treated with heat or hydrated lime. Whenever possible, chop the straw into 3-5 inch pieces with a chopper, lawn mower or string trimmer in a garbage can. Heat pasteurization will kill off most organisms that compete directly with the Oyster mushroom mycelium, and will kill any of the cereal grains present in the straw. Chopped straw should be put into pillow cases, grain sacks, or any similar water penetrating bags. Secure the bags with zip ties or string. Heat a large pot of water to 160°F. Submerge the bags of straw completely in the water, weighing down with bricks or similar objects. Cook the bags for 45-60 minutes, all the while monitoring the temperature (compost thermometers work great for this). Ideally, the cooking temperature should remain around 165°F. Five degrees up or down is fine. When complete, drain the standing water, and cool for about 20 hours or until the internal temperature of the straw is below 75°F.

The Cold Soak Method can be used as an alternative to heat pasteurization. Hydrated Lime (also known horticultural hydrated lime) is a powder made by treating lime with heat and pressure. It is available at garden centers (we use Hi-Yield brand). When added to cold water at a rate of about ⅛ cup (12g) per gallon of water, this soaking solution will make the straw a suitable substrate for Oyster mycelial growth. At this rate, the pH level should be up around 12, which is ideal. It is a good idea to invest in pH strips (litmus paper) to assure you are achieving this level. Adjust the given rate as needed. Add the powder to the soak water, then add the straw. Soak for 18-20 hours, and drain for several hours until the moisture content is around 65%. The squeeze test can be used to estimate moisture content. Simply grab a handful of straw and squeeze as hard as you can. No more than one drop of water should come out.

2. Inoculate
Once prepared, the straw can be inoculated (planted). This process should happen indoors in a clean area with minimal traffic. Use 5-10% of the wet weight of the straw in grain or sawdust spawn: a 20 lb. bag of wet straw would need 1 lb. of grain when inoculated at 5%. A 4 lb. bag of our grain spawn will inoculate roughly ⅔ of a small square straw bale when using a 5% rate (filling about 3 or 4 of our 18 x 36 inch Oyster bags). The straw should be emptied from the soaking bag into a large sterilized container; a stainless steel tub sink that has been disinfected works well. Evenly mix in the proper amount of grain spawn with rubber gloves or clean hands. Tightly and as compact as possible, stuff the straw/spawn mix into polyethylene sleeves, buckets or any other suitable container, seal (with zip ties, string, tape etc.) or cap, and label. Alternately, straw and spawn can be layered within sleeves/buckets, skipping the need for mixing first in a sterilized container. If reusing buckets, be sure to clean thoroughly between uses. Holes for circulation and eventual growth should be put in immediately after planting (an arrow broadhead attached to the end of a wooden handle works great for poking perfectly sized holes in poly sleeves). Using a diamond pattern, punch holes about 4-6 inches apart from one another (see diagram to right).

Time to plant:

Year round indoors.

Time to fruiting:

2-4 weeks (depending on strain).

When they fruit:

Year round indoors.

Grow on:

Straw, cotton seed hull, and other agricultural by-products.

Amount of straw:

One small square straw bale will require approximately 6-8 lbs. of spawn.

Appearance:

Broad color range of white to brown, grey to blue, yellow and pink. Flat thin cap with gills running down an eccentric stem.

Flavor and texture:

Tender, with a mild seafood-like flavor.

Find the Spawn:

Oyster Mushroom Grain Spawn
Please note that King Oyster and Black Pearl Oyster are only suitable for cultivation on sawdust-based substrates.

How to grow Oyster Mushrooms on straw and substrates (1)

3. Incubate
Place filled sleeves/buckets into incubation. Ideally, the room used for this should be somewhere between 75-77°F with a humidity of 80%. During this period, it is extremely important to monitor internal bag temperatures. These temperatures usually peak after 3-5 days of inoculation, and should not exceed 90°F. Spawn death occurs at 100°F. If temperatures become too high increase air flow, decrease room temperature, or relocate your bags/buckets. Bags/buckets should stay in incubation until pinning occurs, usually within 3-5 weeks.

4. Fruiting and harvesting
Once pinning is visible, the bags/buckets are ready to move into a fruiting room. This room should be cooler in temperature, 65°F is optimal. Humidity should be increased to 85-90%. Make sure the bags/buckets receive plenty of light. Generally, 10-12 hours of natural, incandescent or fluorescent is optimal. However, if growing Golden Oysters, it is important that the light be 600 lux: 2× the intensity of normal lighting. The bags/buckets will fruit many times and can last several months. As the number of fruitings increase, yield and quality will decrease.

How to grow Oyster Mushrooms on straw and substrates (2024)

FAQs

How to grow Oyster Mushrooms on straw and substrates? ›

Oyster mushroom cultivation on straw is a popular way to grow an abundant harvest of these delicate mushrooms. The first step in this process is to choose suitable straw for the process- below we'll offer a few guidelines on what to look for.

Can I grow oyster mushrooms on straw? ›

Oyster mushroom cultivation on straw is a popular way to grow an abundant harvest of these delicate mushrooms. The first step in this process is to choose suitable straw for the process- below we'll offer a few guidelines on what to look for.

What is the best substrate for oyster mushrooms? ›

Oyster mushrooms can grow on a ton of different materials, including hardwood sawdust, supplemented sawdust, straw, the masters mix, coffee grounds, paper, and pretty much any other liginous material. The two most common substrates are hardwood sawdust and straw.

How do you sterilize straw for oyster mushrooms? ›

Peroxide Treatment of Straw Substrate
  1. Soak straw until thoroughly wet. Drain. ...
  2. Soak straw in peroxide solution: 1 liter 3% peroxide to 1 gallon. Or 0.6% final concentration.
  3. Keep straw submerged in peroxide solution 24 hours. ...
  4. After 2nd rinse and drain, immediately inoculate straw with spawn.

What is the substrate for straw mushrooms? ›

Straw is an inexpensive material that's readily available in most areas. It makes a good substrate for growing mushrooms because it's easy to break down and full of nutrition. Straw supports nice mushroom fruiting. It's simple and easy to cut up and pasteurize your straw.

Are oyster mushrooms better in sawdust or straw? ›

The oyster mushroom substrate usually uses sawdust. Oyster mushroom producers usually use sawdust as a planted media. Alternatively, rice straw has the same nutrients as sawdust. Rice straw contains 27% hemicelluloses, 39% cellulose, 12% lignin, and 11% dust.

What is the best grain for oyster mushrooms? ›

Brown rice gives your mycelium a tiny bit more nutrition, and it's way easier to see mycelium growing, so that's why we recommend it. You can also use a bunch of other interesting grains like popcorn, birdseed and rye berries. For getting started though, you really can't beat wholegrain brown rice.

How to prepare oyster mushroom substrate? ›

Straw & Cardboard Substrates

To do this, simply submerge the straw or cardboard in very hot water – between 65°C-80°C (or 149-175 Fahrenheit) for up to 2 hours. If using a mushroom growing bag, you can also pour the water directly into the bag, seal it shut with tape or clips and let it sit for up to 8 hours.

What is the best container for growing oyster mushrooms? ›

You don't need much to begin your oyster mushroom growing journey. I'll explain how to grow them in a bucket, but you can always substitute whatever container you have handy — laundry baskets with holes, ice cream tubs, and even trash bags with holes made using a hole punch can work great for growing oyster mushrooms.

Are coffee grounds good for oyster mushrooms? ›

However, this huge amount of waste coffee is still packed full of nutrients which Oyster mushrooms love to grow on. Oyster mushroom mycelium growing on coffee grounds.

Why is lime added to mushroom substrate? ›

Lime works by rapidly altering the pH of the water, making it extremely alkaline and killing off the contaminants in the substrate.

What is the biological efficiency of oyster mushrooms on straw? ›

The biological efficiency was found between 54.5-130.9%, with the moisture of 93%. It was found that C, P, N, and K were integrated into mushrooms with these elements than in the utilized substrate. In DPPH results, the minimum concentration was 37.07 µg/ml, and the maximum was 67.2 µg/ml.

What temperature do straw mushrooms like? ›

Paddy straw mushroom is a warm temperature loving mushroom, hence can be cultivated in the temperature range of 28 to 38ºC and relative humidity (RH) of 57-60%. Ideal temperature for spawn run is 30-35ºC, while 28-32ºC for fruiting. RH of 80-85% is ideal for both spawn run and fructification.

What is a super substrate for mushrooms? ›

Mushroom Growing

Substrate prepared specifically for growing mushrooms is a blend of natural products. Common ingredients are wheat straw bedding containing horse manure, hay, corn cobs, cottonseed hulls, poultry manure, brewer's grain, cottonseed meal, cocoa bean hulls and gypsum.

What mushrooms can grow on straw? ›

You can cultivate many different types of mushrooms on straw such as enokitake, the garden giant, certain Agaricus species, and oysters.

What can you grow oyster mushrooms in? ›

The most widely used substrate for oyster mushrooms is straw. However, sawdust, cardboard, coffee grounds and other byproducts of agriculture such as sugarcane bagasse, coco coir and cotton waste can also be used.

What type of plastic can oyster mushrooms eat? ›

Even for the plastic waste in your own home, oyster mushrooms are coming to the rescue. Pleurotus ostreatus and Pleurotus pulmonarius — both types of oyster mushrooms — were found capable of degrading PET (Polyethylene Terephthalate) plastic over 30 to 60 days.

Will wine cap mushrooms grow in straw? ›

Choose your location and growing medium: Find places in your garden that will receive consistent moisture so that the mushroom mycelium doesn't dry out. Partially shaded areas are OK. Wine caps can be planted in woodchips or straw.

References

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