How To Use Mushroom Grain Spawn - FreshCap Mushrooms (2024)

Growing mushrooms starting from ready-made grain spawn is one of the most accessible and beginner friendly ways to get into the hobby of mushroom cultivation.

It is slightly more complicated thangrowing mushrooms from a kit, but the results are totally worth it!

You’ll get way bigger yields, a deeper understanding of the process, and the ability to grow more mushrooms than you’ll know what to do with.

Why Buy Grain Spawn?

You could always make your own grain spawn…

Without a doubt, if you plan on really diving into the hobby of growing mushrooms,making your own spawnwill be an inevitable part of the process.

It involves properly hydrating and pressure sterilizing grain, inoculating it with your chosen mushroom culture and expanding the spawn through a series of grain to grain transfers until you have a good amount of clean, fully colonized spawn.

This process sometimes causes new growers to shy away.

It requires a pressure sterilizer, a clean laboratory environment, and the skills to properly transfer cultures and grain while staving off contamination. Really, it’s not all that bad- totally doable for people willing to invest a little bit of time into learning the techniques.

But what if you want tojust start growingloads of mushrooms without putting on the lab coat?

That’s where commercially produced grain spawn comes in!

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Types of Grain Spawn

Grain Spawn will typically be made from either Rye, Millet, Sorghum, or a combination of the three. It is probably available in any species you would like to grow – although the commonly available strains are varieties of Oyster (Blue, Pink, Yellow, King, Pearl), Shiitake and Lions Mane.

Spawn is a living, breathing organism, basically a culture of myceliumon the run.It wants to keep running; to keep growing and expanding, colonizing substrates and eventually fruiting. As such, spawn is best whenfresh.

If you are lucky, you live near a spawn producer and can just go pick up fresh spawn. Unfortunately for most people, grain spawn isn’t the type of thing that you can just go pick up at your local market.

There are, however, a number of places that will ship fresh spawn right to your door. Common species will be ready to go and in stock, and less common varieties may take a week or three to prepare before being shipped.

It’s best to choose thefastest shipping optionwhen ordering spawn online- and be quick to pick it up! The less time the spawn spends in transit, the more likely that the spawn will be viable and ready to go when you receive it.

Check with your supplier to ensure that your chosen species, shipping distance and time of year are compatible before ordering.

Caring For Grain Spawn

You should be ready for your grain spawn before it arrives. The quicker you use it, the better your chances of success. Depending on the growing method you choose, this means having a bulk substrate ready for sterilization or pasteurization, and having a fruiting container with a place to fruit it.

This could be outside (at the right time of year), or inside in a growing room.

Inspect your grain spawn on arrival. It should be white and clean, with no visible signs of contamination. If for some reason you see green mold on the inside of the bag, it may not even be worth opening.

With some species, there will be natural excretions, such as the orange metabolite sometimes released by Oyster mushroom mycelium. This is not an issue and won’t affect your grow.

Some species will start to pin on the grain spawn itself. Lions Mane and Pink Oyster mushrooms will commonly pin early. This is usually not a problem, and the spawn can just be used as normal. If the pins are large then it is worth removing them and tossing them aside before using your spawn. This is because the old fruit bodies can be sources of contamination when colonizing a bulk substrate.

If you can’t use your spawn right away, try storing it in a cool, dry and dark location. Most species will do well in a refrigerator, although you have to be careful with tropical species, such as the Pink and Yellow Oyster, which may die off in the fridge.

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Growing Mushrooms on Straw

Growing mushrooms on pasteurized straw is probably one of the easiest and most effective ways to grow mushrooms using commercially produced grain spawn. We have already written a guide for growing mushrooms on straw –check it out here!I’ll summarize the process below.

What You’ll Need

Aside from the spawn itself, you’ll need a few materials and tools to get the job done. Basically, you need to get straw, pasteurize it, and mix in the spawn with a fruiting container. There are countless ways to achieve this goal- feel free to get creative!

  • Straw. Either available in 40lb bails from a local farm, or you canbuy EZ-Straw online. EZ straw will be cleaner and easier to prepare than a square bail
  • A pasteurization method. The best way is to do this outside using a 55 Gallon Drum or similar and apropane burner. Alternatively, you can pasteurize small batches of straw indoors using a large tote, filling with the hot water from your tap, and adding boiling water when needed.
  • A Fruiting Container. This is where you can really get creative. The simplest and most common is to usepoly tubingwith holes cut in the sides, but you can use 5 gallon buckets, totes, laundry baskets… you get the idea.

Basic Steps

  1. Start by pasteurizing the straw, which has been precut into 1-3” pieces. Soak the straw in a hot water bath for 1.5 hours at 65-82 degC. It helps to have a thermometer that you can stick in the water to monitor the temperature. Make sure that you start the timeafterthe water has already reached pasteurization temperature. The 55 gallon drums take a while to reach 65 degC, even with a powerful burner.
  2. Remove the straw from the hot water bath and allow it to drain for 10-20 minutes. The straw should be moist, but not dripping wet.
  3. Spread the straw out on a clean surface to allow it to cool. Adding the spawn to hot straw can kill the mushroom!
  4. Once the straw is cool to the touch, add your spawn! Go for a 10-20% ratio, ie. If you are making a 25 lb log, use 2.5-5 lbs of spawn. This is probably higher than required, but helps increase your chances of success.
  5. Pack your straw and spawn into a poly tubing log. The size of the log is up to you but I wouldn’t go bigger than 16” lay flat diameter which makes about 10” diameter log. You can either mix you spawn and straw together in a large tote then stuff together, or add spawn as you build your log by stuffing a bit of straw, sprinkling spawn ect. until the log is packed.
  6. Cut slits in the bag to allow air the spawn to breath. Place your log in a grow room, or similarly cool and humid environment. The mycelium will colonize the log, and eventually mushrooms will grow through the holes

NOTE: You can get creative here. Five gallon buckets work great with holes drilled in them. You can even use laundry baskets or garbage bags. Growing mushrooms outside in this way -from spring through to fall- works really well, depending on the species. So no need to build a grow room if you aren’t looking for perfection and just want to grow mushrooms!

Growing Mushrooms on Sawdust

Growing mushrooms on supplemented sawdust from grain spawn is a little more complex than growing on straw, but many species, such as Shiitake, Lions Mane and Reishi grow much better on wood than on straw. Definitely worth learning! Check out our article ongrowing gourmet mushrooms on sawdust, or read the summary below.

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What You’ll Need

Once you have the spawn, you’ll need a few extra materials and tools to get the job done. Some are optional, but will make the job a whole lot easier.

  • Hardwood Sawdust. You can use locally sourced hardwood sawdust, or a much easier option, usehardwood pellets. The key is that you use hardwood, such as maple, beech and oak. Softwood won’t work nearly as well, if at all. You can also use supplementation for added nutrients, such as oat or wheat bran
  • Grow Bags. To be more specific, useGusseted Autoclavable Polypropylene Filter Patch Bags. These are special bags made for mushroom cultivation that come in all shapes and sizes.
  • Pressure Sterilizer. You need to pressure sterilize supplemented sawdust, as simply pasteurizing the substrate will lead to contamination. At home, it’s best to use astovetop pressure cookerthat can hold your bags and reach at least 15 PSI.
  • A Flow Hood. To be fair, you don’tneeda flow hood, but it makes things way easier. What you do need, however, is a clean environment to open your bag post sterilization and add the spawn. You can try using a simple Still Air Box, which can often work quite well. Learn all about flow hoodshere.

Basic Steps

  1. Combine water, hardwood sawdust or pellets and supplementation in the form of bran. Mix well and place in grow bag.
  2. Pressure sterilize the grow bags for 2.5 hours at 15 PSI in order to kill off any contaminants present in the substrate. Skipping this step will definitely lead to failure.
  3. Remove the bags and allow them to cool down overnight. Then, in a clean environment (such as in front of a flow hood or in a SAB) add the grain spawn. Use the same ratio of 10-20% spawn to bulk substrate. Seal the bag and mix well by shaking it around. Try to get an even distribution of spawn
  4. Set the bags on a shelf at room temperature for a number of days or weeks until the substrate is completely colonized. Check the whole block over to ensure that there is no left over substrate that hasn’t yet colonized before opening the bag.
  5. Once fully colonized, either cut off the top of the bag, or cut slits in the side of the bag and place it in a fruiting environment. Proper grow rooms, orshotgun fruiting chamberswork best for fruiting blocks. How you decide to fruit the block will depend highly on the species of mushroom you are trying to grow.

Grow In the Garden

Depending on the species of mushrooms you are trying to grow, you may have some success by just mixing the spawn into a substrate in the garden without any pasteurization or sterilization at all. Keep in mind that success from this method is not at all predictable, although you can sometimes get great results with very little effort.

What You’ll Need

  • Straw or Hardwood Sawdust. The idea is to make a bed of substrate.
  • A corner of your yard or gardenthat doesn’t get too much direct sun and can remain moist for much of the day.
  • Ahose,sprinkler, or a good forecast forrain!

Basic Steps

  1. Pick a corner of your yard or garden that is protected from the sun. Best if surrounded by trees or bushes.
  2. Lay down a bed of straw, hardwood sawdust or other similar substrate. Do not use supplementation with this method, as it will just mold.
  3. Mix in liberal amounts of spawn. Mix in well, and try not to leave large amounts of spawn on the top layer, as it will dry out easier. Cover in a thin layer of extra substrate.
  4. Soak the spawn/substrate mixture well. The idea is to keep the area moist, but not completely water logged.
  5. For a period of a few weeks to a month, keep the area moist. The spawn should grow throughout the substrate. Eventually, when conditions are just right, the mushrooms should fruit. Typically this will occur in the fall.

NOTE: When growing with this method, you could have any number of different mushroom species appear in your garden bed. Do not eat a mushroom unless you are confident that you know what it is!

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Get Growing!

That’s it! Of course, there are a million different ways you could try growing mushrooms from spawn, so feel free to experiment with different methods and tactics.

For more ideas on how to use your spawn, or for information on growing mushrooms in general, check out one of these great books:

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Posted by

Tony Shields

Hi' I'm Tony. I've dedicated a ton of time to learn all about the amazing world of mushrooms- and would love to share that information with you! I am particularily passionate about growing mushrooms, and teaching others how to do the same.

How To Use Mushroom Grain Spawn - FreshCap Mushrooms (2024)

FAQs

How do you use grain spawn mushrooms? ›

How do I use grain spawn?
  1. Indoors, you can mix grain spawn with bulk substrate to grow in bags, in containers, or to create fruiting cakes.
  2. Outdoors, you can mix grain spawn with straw or wood chips to make beds and containers.

How long to soak grain for mushroom spawn? ›

The first step to making grain spawn is to soak some cereal grain. Soak the grain by simply placing it in a bucket or bowl, covering in water, and leaving it for 12 -24 hours. If you leave the grain for too long, it will eventually sprout- so try not to soak it for more than 24 hours or so.

How much grain spawn for a 5 gallon bucket? ›

You want to use a spawn rate of around 5-10%, which means you'll need about 2.5-5 lbs of grain spawn for each 5 gallon bucket. A 10% spawn rate will colonize the bucket faster, and comes with a higher chance of success- but you can easily get away with less.

How long can you keep mushroom grain spawn? ›

Grain Spawn should arrive double bagged. Remove the outer bag upon receiving. Keep spawn at room temperature if you will be using it in the next few days. For longer storage, refrigerate the spawn for up to 3 months.

How long to sterilize mushroom grain spawn? ›

Using a pressure cooker or autoclave, sterilize the grains at 15 PSI for at least 90 minutes. You need a good quality pressure canner that can reach at least 15 psi and is large enough to fit your grain jars.

Can you refrigerate grain spawn? ›

Grain spawn will store for three months in a refrigerator. The fresher the better, but mycelium is pretty resilient. If you don't get around to inoculating right away, tuck it away in your refrigerator until you're ready.

What happens if grain spawn is too wet? ›

If your grains are overhydrated, the vermiculite will assist by soaking up excess moisture instead of pooling at the bottom of the jar smothering the grain. Excess water pooling at the bottom of the jar or bag is a breeding ground for bacterial endospores. So it's best to dump jars like this out and start over.

What is the best temperature for mushroom grain spawn? ›

The ideal temperature for a spawn run depends on the type of mushroom, but it is generally between 23 and 28 Celsius (73 and 82 Fahrenheit).

How long does it take for grain spawn to fully colonize? ›

Colonization. Colonization occurs after inoculation. This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions.

What is the best ratio for grain spawn to substrate? ›

The more spawn used the faster the bulk substrate will colonize. Recommended spawn ratios are usually no less than 20% (1 part spawn to 4 parts substrate).

What size bags for grain spawn? ›

In my experience, the large sized bags are by far the most useful. To give you an idea, a bag of size 8 inches wide by 5 inches deep by 18 inches tall will comfortably hold 5 lbs of supplemented sawdust and grain spawn.

How much liquid culture to add to grain spawn? ›

Usually about 1 – 2 CC's of liquid culture is sufficient per quart sized grain jar. You can also inoculate the grain jar without even opening the lid, by simply injecting the liquid culture right though the filter on the top of the jar.

What to do with leftover grain spawn? ›

Sawdust spawn, grain spawn, and even plug spawn can be used to inoculate the blocks you make. If you are new to making your own blocks, we recommend you use a heavy inoculation rate (think at least 1 cup of spawn per block).

How to use mushroom grain spawn? ›

Drain off excess moisture and when cool enough to handle mix grain spawn throughout at a ratio of 1:5 up to 1:10 and load into your chosen fruiting vessel (e.g a bucket with holes, mycobag or plastic bag). A Pressure Cooker, or large vessel to steam the bags in, such as 200L drum.

How do you know if mushroom spawn is bad? ›

Spend time getting to know what your mycelium is supposed to look like so you can better identify unhealthy or contaminated patches. While mycelium is typically white and filamentous, contamination generally takes the form of green, blue, gray, or black patches or discolorations in your substrate.

Why use grain for mushroom spawn? ›

Grain is highly nutritious- full of starch and sugars. When properly hydrated, it serves as an ideal food source for hungry mushroom mycelium. Unfortunately, this also an ideal growing environment for mold and bacteria- which in most cases can grow much faster and stronger than mushroom mycelium.

When to mix up grain spawn? ›

You can shake up your mushroom spawn bag when the mycelium has colonized about 40–50 percent of the spawn grain.

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