Storing liquid culture
Liquid culture is best stored in the fridge at 2-4 ° C once the syringes arrive.
The syringes can be stored unopened in the fridge for up to 3 months.
Liquid culture syringes that contain Pink Oyster mycelium should not be stored in the fridge.
Pink oyster mycelium do not fare well is stored cold long term.
How much liquid culture should I use?
How much liquid culture you need is depending on the medium you are inoculating.
If you are using our sterilised grain bags we recommend that you use 10 ml of liquid culture per bag.
You can use less but it will slow down the process while increasing the risk of failure.
It is possible to reuse the same syringe on multiple occations.
If you still have some liquid culture left in the syringe when you are done inoculating you can add the protection cap back on the needle and reuse the parafilm to secure the plunger and the protection cap.
Store the leftover liquid culture in the fridge and use it within 2 weeks.
When you are ready to use the liquid culture again please scrub down the whole syringe with rubbing alcohol.
Please also heat up the needle itself with a lighter until it is glowing read to sterilize it. You never know what kind of bacteria can be growing in the fridge.
Can I add liquid culture directly to my substrate?
It is possible but we do not recommend it. This since the chance of failure is bigger.
We recommend that you use the liquid culture to inoculate grains and use the grain spawn to inoculate your growth substrate. Whether it is sawdust, hay or cardboard.
Just break up the grain spawn once the mycelium have taken over it. Then inoculate the growth substrate you want to use.
The structure of the grains give the mycelium more points of attack to spread from in the growth substrate compared to just spraying liquid culture in it.
So this speeds up the incubation period and reduces the risk of failure.
