Inoculating Logs - Cornell Small Farms (2024)

EXPERT OPINION: Inoculation Methods

“Sawdust spawn allows the inoculator to work faster than with a dowel and hammer. Also, I believe that sawdust spawn leaves less room for contamination because the spawn is not touched by your hands.”

- Nick Laskovski, Dana Forest Farm

EXPERT OPINION: Strain Choice

“Most growers in cooler climates tend to use Wide Range strains in the majority of their logs. I recommend that people always start here and then branch out into the WW and CW strains.”

- Steve Gabriel, Wellspring Forest Farm, Mecklenburg NY

EXPERT TIPS: Cold Weather Strains

“I’ve been using the cold weather strain Snowcap for several years (from Field and Forest), and have been harvesting them for more than two weeks (they started pinning in early April) - with no cover. They sailed right through the last cold snap (low-20s, daytime temps not much above freezing) and are coming on strong now.

“I am shocking 20 logs per week of those that didn’t ‘wake up’ naturally, and they are also pinning happily now.

“For people in the North Country like me (20 miles from Canada/we get below-40-degree nights right into June, and again by late August), you might consider diversifying your strains.

“I highly recommend SNOW CAP, and also BOLSHOI BREEZE. Both can keep fruiting right into November - and tend to pick up when the WR and Warm-weather strains poop out.”

-Robert Wagner, North Woods Shiitake

Inoculating Logs - Cornell Small Farms (1)

Bag of sawdust spawn and inoculator (Allen Matthews, Chatham University).

Inoculating Logs - Cornell Small Farms (2)

Plug spawn being placed in a hole (Jim Ochterski, Cornell University).

Spawn Type

Bolts can be inoculated with sawdust spawn, plug spawn, or thumb spawn. There are advantages and disadvantages to each. Plug spawn is very simple to use—the only tool needed is a drill and hammer or mallet. The drawbacks are that plug spawn is slightly more expensive per log than sawdust spawn, the mycelium may take a while to migrate into the sapwood of the log, and the inoculation operation is significantly slower than the sawdust spawn method. Sawdust spawn is slightly cheaper than plug spawn, but it requires a special tool (inoculator), either purchased or homemade, to insert the sawdust into the hole. In addition to its lower cost, the mycelium contained in sawdust spawn moves out into the log more quickly, giving somewhat faster produc- tion. This guide specifically describes the sawdust spawn method, which is more common for commercial enterprises.

Choosing Spawn Strain

Mushroom supply catalogs describe strain categories that differ in the tem- perature range at which they fruit. Each category described below includes several strains (varieties) of spawn and each of these is a genetically unique clone. The differences between each strain temperature category are also summarized below. Generally speaking, we recommend trying a variety of different strains to see what works best under your specific conditions. By us- ing a combination of strains, many growers are able to maximize the length of their fruiting season.

People sometimes wonder if there is a taste difference between different strains. Although there isn’t data on the subject, commercial growers tend to find that cold weather strains produce shiitake with a more intense flavor. Try a few strains and see what you think. If you intend to try only one strain the first time around, we recommend a WR strain.

  • Wide Range (WR) Strains: These are the favorite among both commercial growers and beginners. They are reliable. Fast spawn run and fruiting al- low a faster return on investment. WR strains can be force fruited easily and recover vigor more rapidly after fruiting.
  • Warm Weather (WW) Strains: Warm Weather strains produce the highest quality mushrooms during the hot summer months and early fall. They respond well to soaking for commercial production, and tolerate warmer soak water than other strains. They are also often recommended for grow- ing Shiitakes on some of the softer hardwoods, such as red maple.
  • Cold-Weather (CW) Strains: These tend to fruit in early spring and late fall. These strains require a longer spawn run than other stains. They won’t re- liably fruit after being forced, so most people let the natural rise and fall of wintry temperatures initiate fruiting. See side-bar for more information about cold weather strains.

Using Cold Weather Strains to Extend Season

Cold weather strains are well suited to the Northeast where the growing season is short and the time frame for fruiting wide range and warm weather Shiitake strains can be limited. Since cold weather strains fruit during the transition times from spring to summer and summer to fall, they can be used as a strategy for season extension. Even though cold weather strains do not respond well to shocking, Northeast-based growers with enough cold weather strain logs can supply late spring and fall markets when other mushroom growers can’t. Learn more about season extension using cold temperature strains on page 31.

Log inoculation is a three step process. First a series of small holes are drilled into each log. Each hole is then tightly packed with spawn. Lastly, the surface of each hole is covered with wax to ensure optimal mycelium growing conditions and reduce contamination by other fungi species. Each step is described in detail on pages 20 through 22.

This process can be quite repetitive and time consuming; however, many growers have found innovative ways to speed it up. For example, you can solicit volunteer help by hosting an inoculation party or informal mushroom inoculation class; see page 17 for more details. You may also want to consider setting up a small assembly line. Building an inoculation table is a great way to save time and your back. Ideally, you will want a table that keeps the log from rolling over when you are working with it, but also makes it easy to move the log from one station to another. Some handmade inoculation tables are pictured below to offer inspiration.

Selecting Appropriate Spawn

Inoculating Procedure Using Sawdust Spawn

Inoculating Logs - Cornell Small Farms (3)

Homemade inoculation table. (Ross Conrad, Dancing Bee Gardens).

Inoculating Logs - Cornell Small Farms (4)

Homemade inoculation table at Red Manse Farm, NH (Allen Matthews, Chatham University).

Inoculating Logs - Cornell Small Farms (5)

A black-and-white illustration of the inoculation process from drilling to waxing. Drawing by Carl Whittaker.

Inoculating Logs - Cornell Small Farms (2024)

FAQs

How long does it take to inoculate logs? ›

Logs can be inoculated immediately after they are felled; there is no need to “cure” the log for a few weeks. Do not wait longer than three weeks to inoculate; the longer you wait, the more likely the log is to be infested with competitive fungi and/or dry out.

What is the method of oyster mushroom cultivation? ›

Oyster mushrooms are grown from mycelium (threadlike filaments that become interwoven) propagated on a base of steam-sterilized cereal grain (usually rye or millet). This cereal grain/mycelium mixture is called spawn and is used to seed mushroom substrate.

What is the mushroom cultivation system? ›

Mushroom farming consists of six steps, and although the divisions are somewhat arbitrary, these steps identify what is needed to form a production system. The six steps are Phase I composting, Phase II composting, spawning, casing, pinning, and cropping.

Which mushroom is best for farming? ›

One of the most popular types of mushrooms to grow is the button mushroom. These are the most commonly consumed mushrooms in the world and are relatively easy to cultivate. They grow best in a temperature range of 55-60°F and require a substrate made of composted manure, straw, and gypsum.

How long after inoculation should I see mycelium? ›

After inoculation, the spawn needs time to grow through the substrate. This process is called incubation. Incubation starts with leap off, which is the moment when the mycelium initially leaps off the spawn onto the new substrate. This typically occurs around four days after inoculation.

What is the best temperature to incubate mycelium? ›

Growing mycelium should be kept in an ideal temperature range. For example, P. cubensis colonizes most rapidly between 75-80°F (24-27°C). Temperatures higher than this range may kill the mycelium and encourage growth of contaminants, and temperatures lower than this range may slow down colonization.

What are the problems with mushroom cultivation? ›

The challenges faced by mushroom growers include inadequate supply of spawn at the appropriate time, unfavorable climatic conditions, lack of cold storage facilities, poor marketing avenues, and the perception of mushrooms as non-vegetarian food.

What is a mushroom farmer called? ›

Most mycologists work in academia; government research labs; or industries such as biotechnology, biofuels, and medicine. However, there are also opportunities in areas such as mushroom farming; mushroom bioproducts, such as packaging materials and leather alternatives; and foraging.

Which season is best for mushroom cultivation? ›

It can also be cultivated in summer months by providing the extra humidity required for its growth. In hilly areas above 900m. (m.s.l.), the best growing season is during March/April to September/October and in the lower regions from September/October to March/April.

What is the most profitable mushroom to farm? ›

The most profitable mushroom to grow can vary depending on market demand, local preferences, and cultivation costs. Growing oyster mushrooms like Shiitake are usually the most profitable.

What mushroom is worth a lot of money? ›

French Black Truffles

French Black Truffles are one of the most expensive mushrooms, and grow in various regions of France. However, you can also find French Black Truffles growing in southern areas of Europe. Currently, these mushrooms are sold in United States retail markets and go for $1,000 to $2,000 per pound.

How fast does mycelium colonize? ›

Colonization occurs after inoculation. This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions. Ideal conditions for this phase are in a dark place and temperature between 72 F – 80 F.

Can you inoculate mushroom logs in summer? ›

While inoculating trees downed in the summer is not usually recommended it can, in fact, yield a respectable amount of mushrooms.

Can I inoculate old logs? ›

The answer is no. The window of inoculating hardwood logs is anywhere from 1 week to 6 weeks after cutting. In cooler climates, some growers cut logs in the fall, stack, and cover them with a tarp to overwinter, then inoculate in the spring.

How do you inoculate a log? ›

Inoculating logs in 5 steps
  1. Drill your holes. Use your 5/16″ bit, set to approximately one inch depth using a bit stop or a band of duct tape. ...
  2. Insert your mushroom plugs. Tap your plug spawn into the holes using a mallet or hammer. ...
  3. Seal them in. ...
  4. Set your logs up. ...
  5. Keep them happy.
Feb 11, 2021

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