Mushroom Growing Substrate: Complete Guide (2024)

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Growing mushrooms starts with selecting the right mushroom growing substrate for your grow. The mushroom growing substrate is another word for the mushroom growing medium. This is what the mushroom mycelium grows through and it provides nutrition to the mushroom mycelium and subsequent fruiting mushrooms.

Mushroom Growing Substrate: Complete Guide (1)

You can think of a mushroom growing substrate just like soil for plants – some plants require special soil or enriched soil, while others don’t. In this guide, we will break down the different substrates that common growing mushrooms prefer.

Choosing a Mushroom Growing Substrate

The key part to understand is that every mushroom tends to grow best in certain types of substrates. For example, a King Oyster mushroom may grow best in hardwood sawdust, while a White Cap mushroom may prefer Manure.

As stated above, the nutrition of each substrate is what the mushrooms depend on to grow. This can be protein content, sugar content, potassium, fats, and nitrogen.

Another key component of the mushroom growing substrate is the water content. Many substrates require water as an added ingredient, but the amount of water is important. This can sometimes be 1/4 water as the volume of the ingredients, but it highly depends on the mushroom chosen.

You may have learned from other sources that sterilization of the substrate is important, and this cannot be understated. Using a sterilized growing medium is extremely important, otherwise you risk mold overtaking your substrate and ruining the mycelium. Always use safe and effective methods to sterilize your mushroom growing substrate, such as a pressure cooker.

Learn how to sterilize substrates in our guide here.

Mushroom Growing Substrate Examples

King Oyster Mushroom Substrate

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King Oyster mushrooms grow best on cellulose-rich mediums. This is usually going to be sawdust, straw, or a mix of the 2. Our best recommendation for king oyster substrate is the following:

  • Blend of hardwood sawdust (Amazon)
  • Supplement of 5-10% of wheat bran (Amazon)
  • 60-70% moisture content
  • a ph level of 6.5 after sterilization.
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These conditions and ingredients will allow the king oyster mushrooms to grow best. Other mediums can prove successful, but this blend is known to be highly effective.
Always take into consideration the other aspects of a well-controlled grow such as temperature of the grow room and lighting. While we can’t cover all of that detail in this guide, we have plenty of guides on that information on our site here.

Shiitake Mushroom Substrate

Mushroom Growing Substrate: Complete Guide (4)

Shiitake mushrooms grow best on wood-based mediums. Much like the king oyster mushroom, they prefer hardwood sawdust. Other mediums will work, but Shiitake mushrooms are more picky than Oyster mushrooms and it’s best to choose sawdust or other forms of ground wood. Here are the ingredients for Shiitake substrates:

  • Blend of hardwood sawdust (Amazon)
  • Supplement of 0-5% of wheat bran (Amazon)
  • 55% moisture content
  • a ph level of 6.5 after sterilization.

Portabello Mushroom Substrate

Portabello mushrooms prefer an alternative substrate of manure-based compost (Amazon). This will usually be a compost that is blended with manure. This should be easy to find, as many types of compost already come mixed with manure.

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You will still perform the normal steps of sterilization, and layer your manure-based compost into a wide cubic bin. Then, you’ll pour in some of your portabello spores and then leave the substrate to grow. This can be done indoors or outdoors, but given that compost/manure can be a bit messier than other substrates, outside is best.

White Button Mushroom Substrate

White Button mushrooms also prefer manure-based compost (Amazon) as their preferred growing medium. This will be a similar process to the above portabello guide.

An easy trick for growing white button mushrooms is to combine a 50/50 blend of manure and compost and blend it up. Now, with this substrate prepared, you can be assured you’re using the best substrate for white button mushrooms.

Conclusion

We listed a few of the most popular mushroom’s favorite growing mediums. These are some of the mushrooms that are easiest to grow at home. In the entire kingdom of mushrooms, there are so many mushrooms that require such special growing circ*mstances that you simply can’t grow them at home. This includes mushrooms that require a fresh forest burn to grow, or mushrooms that grow on dying trees.

Because of this, choosing easy-to-grow mushrooms is important, especially as a beginning grower. Choose a simple mushroom with an simple substrate and get started.

To really drive the point home, we highly recommend you take the sterilization of your growing substrate very seriously. Much of your hard work can go to waste if you don’t properly sterilize your mushroom growing medium with a pressure sterilization method.

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Mushroom Growing Substrate: Complete Guide (2024)

FAQs

Mushroom Growing Substrate: Complete Guide? ›

The more spawn used the faster the bulk substrate will colonize. Recommended spawn ratios are usually no less than 20% (1 part spawn to 4 parts substrate).

What are the steps in mushroom substrate? ›

Contents
  1. STEP 1: Growing out a chosen mushroom culture on an agar filled petri dish.
  2. STEP 2: Transferring the mushroom culture (known as mycelium) onto sterilized grain.
  3. STEP 3: Expanding the grain jar exponentially to create grain spawn.
  4. STEP 4: Spawning the grain into a suitable substrate and fruiting container.

What is the best ratio for mushroom substrate? ›

The more spawn used the faster the bulk substrate will colonize. Recommended spawn ratios are usually no less than 20% (1 part spawn to 4 parts substrate).

How wet should mushroom substrate be? ›

Your substrate should be slightly acidic, with a PH between 5 and 6.5. (Some mushrooms, such as oyster mushrooms, can withstand a PH of up to 8.) A minimum moisture content of 50-70% is essential for your substrate.

Do you have to sterilize mushroom substrate? ›

The requirement for substrate sterilization depends on the nutritional content and the type of mushrooms one aims to grow. Substrates with a high level of supplementation, such as manure, soy hulls, grains, and master's mix, as well as nutrient-rich substrates, are more prone to contamination.

How long does it take for mushroom substrate to colonize? ›

Colonization occurs after inoculation. This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions. Ideal conditions for this phase are in a dark place and temperature between 72 F – 80 F.

Can you use just coco coir for mushroom substrate? ›

Whether you're a hobbyist or a commercial grower, embracing coco coir as a mushroom substrate can significantly improve the success of your mushroom cultivation activities. As a result, you can enjoy a bountiful harvest yourself and/or contribute to the growing demand for nutritious and delicious mushrooms.

Are coffee grounds a good mushroom substrate? ›

It just makes sense to grow mushrooms in coffee grounds. You make wonderful nutritious oyster mushrooms out of a bountiful waste resource that is still rich with nutrients. You can return the now composted grounds to enrich your soil at the end of the growth cycle to complete its life cycle too.

What is the easiest mushroom substrate? ›

Pretty much every mushroom growing resource I could find says that oyster mushrooms are the easiest variety for first time-growers, as they grow fast and can easily thrive in substrates made of things like coffee grounds and straw, making them relatively low maintenance.

How to tell if mushroom substrate is too dry? ›

A) lots of water dripping shows that the substrate is too wet. b) a few drops released with some pressure show that the substrate is well watered. If it is too dry then with no drops will be released with pressure.

How many times can you reuse mushroom substrate? ›

Yes, you can most definitely reuse mushroom grow kits! Mushroom grow kits are generally designed to give you more than one harvest depending on the species of mushroom inside your growing kit. There is enough water nutrition available in the substrate to give you multiple harvests over a period of 2-10 weeks.

How to know if substrate is too wet? ›

Take some substrate with your hand, squeeze it and observe if any water is released through your fingers while squeezing. There are three different outcomes to this test: Water is running from your hand while squeezing the substrate: This means the substrate is too wet because too much water has been added.

Does mycelium grow faster in the dark? ›

Radial growth of the mycelium was faster under dark incubation rather than under light incubation.

How much lime to add to mushroom substrate? ›

Substrate may also be treated to reduce potential fungal or bacterial contaminants. Two common methods of substrate treatment include: Cold water with hydrated lime: Mix 6 grams of hydrated lime for every 1 gallon of water. You may need to add more or less depending on the pH of your water.

How do you prevent mold in mushroom substrate? ›

How to avoid mould issues
  1. Avoid warm humid weather. Mould loves warm humid weather. ...
  2. Avoid soils and plants. Many moulds are present in all soils. ...
  3. Clean your knife or cutting implement. ...
  4. Avoid touching the substrate and plastic with your fingers. ...
  5. Avoid over-watering. ...
  6. Open your kit as soon as you receive it.
Sep 26, 2021

What are the stages of mushroom cultivation? ›

The six steps are Phase I composting, Phase II composting, spawning, casing, pinning, and cropping. These steps are described in their naturally occurring sequence, emphasizing the salient features within each step.

What are the stages of mycelium? ›

The mycelium (the 'body' or 'roots' of the mushroom) grows through this substrate and excretes enzymes to convert it into an absorbable form. There are four basic stages to the life cycle of a mushroom: Spore germination, colonisation, fruiting, and sporulation.

What are steps involved in the preparation of mushroom fungal culture? ›

It describes the key steps as: preparation of spawn/seed by inoculating wheat grains with fungal mycelium; preparation of the substratum or growing medium by processing materials like rice straw or bagasse; cultivation using the cylindrical block system by layering spawn and substratum in bags; maintaining proper ...

What is the process of mushroom processing? ›

MUSHROOM PROCESSING

Initially mushrooms are washed in cold water then blanched in boiling water for 3‐4 minutes. They are then dehydrated in drier and packed. It is advisable to pre‐treat mushroom in brine solution to prevent discoloration.

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