creating-a-spore-print-mushrooms (2024)

FAQs

Creating-a-spore-print-mushrooms? ›

Put a drop of water on the top of the cap to help release the spores. Cover the cap with a paper cup or glass and leave for 2-24 hours, depending on the humidity and the freshness of the mushroom. The spores will fall on the paper, foil or glass, making a spore print pattern.

Take the stems off the mushrooms using your hands or scissors. Place the mushrooms with the gills or pores facing down onto the paper. Then place the container over the mushroom to create an airtight seal. Leave the mushrooms covered anywhere from a couple hours to overnight based on how fresh the mushrooms are.

White and black paper are commonly used for this purpose – you would make sure half of the mushroom sites over the white paper and the other half sits over the black paper. White paper works well for mushrooms with non-white spore prints, while black paper is ideal for capturing white or light-colored spores.

Remove the stem of the specimen and place the cap of the mushroom on the spore print card with the gills (or pores) facing downward. Cover it or leave it undisturbed overnight and compare the colour of the resulting print with descriptions in your field guide.

The spore print is the powdery deposit obtained by allowing spores of a fungal fruit body to fall onto a surface underneath. It is an important diagnostic character in most handbooks for identifying mushrooms. It shows the colour of the mushroom spores if viewed en masse.

Cultivating spores

Once you have a spore print (by following the instructions above) you can then use it to cultivate and grow your mushrooms. This can be done by creating a spore syringe, where the spores are re-hydrated using sterile water and then used to inoculate the growing medium.

Leave everything where it is for at least 2 hours or longer (overnight). 4. Carefully remove the bowl and the mushroom cap. You should see a pattern on the card that matches the pattern of gills on the mushroom!

Spores may be collected from a spore print, which is a powdery imprint left on a surface by the spores of a mushroom. Spore prints are an important field mark for identifying mushrooms, as their colors can help distinguish between species. A mushroom releasing spores.

Spore prints in a herbarium allow future researchers to examine mature spores, so they need to be reasonably thick if possible. After air drying for half an hour, they should be protected against being rubbed off, eg wrapped in aluminium kitchen foil. It's a good idea to label both the slide and the foil.

Maintaining the ideal humidity is perhaps the trickiest part of making spore prints. Dry mushrooms won't give a good spore print, but wet ones can make a sloppy mess. If you put a small mushroom on a piece of paper and cover it with a large bowl you may end up with a shrivelled cap and no spore print.

How to put spore print in syringe? ›

Then, to make a spore syringe, scrape the spore print into a glass with sterilized water and pull the solution into a syringe. After you have created a spore print, you can save it for later use, make a spore syringe, apply it to an agar solution or create a liquid culture.

To make liquid culture, mix a sugar source (like light malt extract or honey) with water, sterilize the solution, and then introduce mushroom mycelium. Use a canning jar with an airport lid for the culture and a pressure cooker for sterilization.

Then, to make a spore syringe, scrape the spore print into a glass with sterilized water and pull the solution into a syringe. After you have created a spore print, you can save it for later use, make a spore syringe, apply it to an agar solution or create a liquid culture.

5. Lift the jar and remove the mushroom to reveal your mushroom spore print! Allow it to air dry for two hours before storing it in a safe dry place. You can also fram the spore prints in a frame as art.

All you need to do is inject around 1ml of spores into the liquid culture vial, then shake. There's no need for a sterile environment, and you'll cultivate a high-quality liquid culture in return.