How to Create Mushroom Spore Prints (2024)

How to Create Mushroom Spore Prints (1)

Creating spore prints is a fun way to observe spores, they are microscopic and otherwise hard to notice. This is a fun STEM activity for children of all ages. Making spore prints is also what people can do to collect the spores and grow mushrooms. spore prints also help you to identify the mushroom. We may cover germination, fruiting and identification in another blog post, for this blog post it’s all about creating the spore prints!

Going outside on an outdoor forest walk to hunt wild mushrooms is part of the fun. Mushroom hunting is one of our favourite activities because you never know what fungi you’ll find! It’s like a treasure hunt. If you don’t have a forest to hike in, you can often find wild mushrooms anywhere, including your own backyard. Try to stick with edible mushrooms that you are familiar with and if you are unsure, wear gloves and collect the spore prints outside.

Freshly picked wild mushrooms works the best for spore prints because store bought ones may be too old. However, if you can’t find any wild mushrooms, you could give store bought ones a go, but be aware that it may not work, or may be a much lighter colour.

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Materials:

  1. fresh picked wild mushrooms

  2. white paper or black paper (depending on the gill colour and spore colour)

  3. a jar, bowl or glass

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There are only 5 steps:

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  1. Go mushroom hunting and pick some wild mushrooms.

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2. Remove the stem then place the cap (top of the mushroom) gill side down onto a white piece of paper.

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We kept this as one step because after you remove the stem the cap will still be in your hand, so simply place it onto a piece of paper. .

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3. Cover the cap with a bowl, glass or a jar. Anything airtight that will keep the spores from escaping.

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4. Allow the mushroom to sit flat, undisturbed for two to 12 hours. The longer you leave it, the greater the chances of more spores being released, creating a darker print. Don’t leave it any longer of it could start to rot. We generally leave it 2-8 hours.

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5. Lift the jar and remove the mushroom to reveal your mushroom spore print!

Allow it to air dry for two hours before storing it in a safe dry place. You can also fram the spore prints in a frame as art.

If you are allergic to spores, create this activity outside.

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DID YOU KNOW? You can create dark spore prints by using dark gilled mushrooms on white paper. and

You can create white spore prints by collected white coloured gills and placing them onto black paper.

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Some spore prints are orange and various other colours!

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Tips on choosing the right mushrooms:

1. Mushrooms with large caps with flat gills work really well, but avoid any from the Amanita genus.

2. I’ll repeat, Avoid using poisonous mushrooms!

Spore prints help us identify the type of mushroom it is, it can help tell you a poisonous mushroom from an edible one, but if you are creating this with children, you’ll need to avoid these mushrooms entirely.

Common name’s from the Amanita species are death cap and destroying Angel, this is because they are super poisonous and could cause death if you consume it. Chances of getting sick just by touching is isn’t common but can happen if you don’t wash your hand properly. So it’s always important to use caution around these kinds of mushrooms.

The defining features of an Amanita species are:

  1. Button stage when young which can look like a white puffy ball

  2. A Volva (universal veil) attaches to the stem in the ground and looks like a round white bulb.

  3. A ring around the stem (a concentration of the poison resides there).

  4. warts on the cap from remnants of the universal veil.

  5. White gills

  6. They range from red, orange to yellow and a white cap depending on the species.

  7. Can often have a strong odour, ammonia like (but not always).

  8. Has a white spore print.

Here‘s what some Amanita Mushrooms look like, just to make sure you avoid using them!

Button stage: (young growth)

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Adult stages:

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There are countless other poisonous mushrooms but most are only harmful when you eat them and the spores aren’t poisonous. Spores can cause irritation to breathing and lungs but making a spore print is safe because you put an airtight container over the mushroom’s while they are being released. With wild mushrooms it’s always best to be cautious about what you eat but you can still curious and explore them by taking photos, making spore prints and even slicing them open to see what they look like as a sectional. As mentioned previously, you can wear gloves if you are unsure and make your spore prints outside. You can learn a lot from books and by observing the different features.

Never consume a wild mushroom without the advice of an expert.

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We hope that you have fun creating wild mushroom spore prints! If you create this activity and would like to share it with us, feel free to tag us on Instagram at @acorns.and.aprons - we would love to see and share your spore creations!

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FAQs

How to Create Mushroom Spore Prints? ›

Making a mushroom spore print and spore syringe is as simple as placing a mushroom cap with the gill

gill
In mycology, a lamella ( pl. : lamellae), or gill, is a papery hymenophore rib under the cap of some mushroom species, most often agarics.
https://en.wikipedia.org › wiki › Lamella_(mycology)
side down on a sheet of aluminum foil, covering it with a bowl, and waiting for about 24 hours for the spores to drop onto the surface below.

What paper is best for mushroom spore prints? ›

To make a spore print you will need a mushroom with a cap, one piece of white paper, one piece of dark colored paper (black works best), a cup or something similar to cover the mushroom cap, and a safe dry place to set it. find a mushroom with a cap.

How to do a spore print test? ›

Remove the stem of the specimen and place the cap of the mushroom on the spore print card with the gills (or pores) facing downward. Cover it or leave it undisturbed overnight and compare the colour of the resulting print with descriptions in your field guide.

How do you inoculate spore prints? ›

If working with a reusable inoculation loop, heat the inoculation loop red hot in the flame of an alcohol lamp and let it cool down in your hand. Do not touch the spore print, inoculation loop or agar medium. Now rub the spore print until the whole loop has gathered some spores.

Why make a mushroom spore print? ›

The spore print is the powdery deposit obtained by allowing spores of a fungal fruit body to fall onto a surface underneath. It is an important diagnostic character in most handbooks for identifying mushrooms. It shows the colour of the mushroom spores if viewed en masse.

What is the best medium for spore prints? ›

Lay the cap of the mushroom with the gills upside down onto a piece of paper. For the majority of specimens, a normal sheet of white paper works fine. However, some mushrooms have white spores- so if you are taking prints for the purposes of identification, you might want to consider also getting some black paper.

How do you get the best spore print? ›

Put a drop of water on the top of the cap to help release the spores. Cover the cap with a paper cup or glass and leave for 2-24 hours, depending on the humidity and the freshness of the mushroom. The spores will fall on the paper, foil or glass, making a spore print pattern.

How to create spore prints? ›

Take the stems off the mushrooms using your hands or scissors. Place the mushrooms with the gills or pores facing down onto the paper. Then place the container over the mushroom to create an airtight seal. Leave the mushrooms covered anywhere from a couple hours to overnight based on how fresh the mushrooms are.

Why no spore print? ›

Maintaining the ideal humidity is perhaps the trickiest part of making spore prints. Dry mushrooms won't give a good spore print, but wet ones can make a sloppy mess. If you put a small mushroom on a piece of paper and cover it with a large bowl you may end up with a shrivelled cap and no spore print.

What does a mushroom spore print tell you? ›

A glass jar is placed over the cap to contain the mushroom during the printing process. After 24 hours hours, the cap is removed from the paper to reveal spores that have fallen. The color of the spore print can help identify the genus of a specimen.

How long after inoculation should I see mycelium? ›

After inoculation, the spawn needs time to grow through the substrate. This process is called incubation. Incubation starts with leap off, which is the moment when the mycelium initially leaps off the spawn onto the new substrate. This typically occurs around four days after inoculation.

What is the best way to preserve spore prints? ›

Preserving and Storing Mushroom Spore Prints
  1. Fixative or Hairspray: To preserve the mushroom spore print, you can lightly spray it with an artist's fixative or hairspray. ...
  2. Lamination: If you want to preserve the spore print for a long time, consider laminating it.
Nov 2, 2023

What should mycelium look like on agar? ›

If you cultivate mushroom mycelium on agar medium there will appear different forms of mycelium. The two main forms are: rhizomorph mycelium and 'fluffy' mycelium (looks like cotton). For further cultivation and introduction of fruiting only the rhizomorph mycelium is suitable.

How long does it take for a mushroom spore print? ›

Cover the mushroom with a bowl (to stop it drying out and to reduce air currents that might spoil your print by blowing the tiny spores around). 3. Leave everything where it is for at least 2 hours or longer (overnight).

What is the difference between a spore and a print? ›

Spores may be collected from a spore print, which is a powdery imprint left on a surface by the spores of a mushroom. Spore prints are an important field mark for identifying mushrooms, as their colors can help distinguish between species. A mushroom releasing spores.

What is the color of a spore print? ›

In this quantity the colour is easy to see. The commonest spore colours are white and various shades of brown. Note that the colour of the gills need not be the same as the colour of the spores. If your spore print is very weak (or not there) put the cap and tub back down and leave it for longer - even overnight.

What paper is used for mushroom substrate? ›

Toilet paper, although not very rich in nutrients, can support the growth of an incredible mushroom...the oyster. This mushroom can grow on a wide variety of substrates like paper, cardboard, coffee grounds, straw, sawdust, and logs (just to name a few).

How do you preserve spore prints on paper? ›

To preserve your spore print, spray them lightly with an artist spray or hair spray.

What is mushroom paper? ›

This can give them texture, strength, and other properties (for example, comparing foil to printer paper), but nowadays we can use mushrooms which can be a good substitute for wood for paper making. The mushroom paper differs from ordinary paper in that chitin fibers are used instead of cellulose.

How do you seal a mushroom spore print? ›

Take the stems off the mushrooms using your hands or scissors. Place the mushrooms with the gills or pores facing down onto the paper. Then place the container over the mushroom to create an airtight seal. Leave the mushrooms covered anywhere from a couple hours to overnight based on how fresh the mushrooms are.

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