What Is the Best Grain for Mushroom Spawn? (2024)

What Is the Best Grain for Mushroom Spawn? (1) Audrey Ferguson| Updated on: March 27, 2024

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One of the most common methods of growing mushrooms is using something known as grain spawn. In brief, it involves using a cereal grain to provide nutrition to the fungus as its mycelium develops.

There are several options in terms of which grain to use, each with distinct advantages and disadvantages. This article explains how to choose the best grain for mushroom spawn according to your needs. Here’s what you need to know.

What Is Grain Spawn?

Before you can produce a bountiful mushroom harvest, it is necessary to cultivate some healthy mycelium.

Mycelium is the network of strand-like structures called hyphae that make up the bulk of the fungal organism. Much like a plant’s roots, it takes up nutrition from the substrate and grows and develops until the conditions are right for the first flush of mushrooms to form.

Depending on the species, mushrooms can be grown on many different media. Wood chips, coco coir, or even coffee grounds are all popular options for the “bulk substrate,” the substance on which the mushrooms will eventually fruit. However, it isn’t simply a case of throwing some spores onto the bulk substrate in question and waiting for mushrooms to grow.

What Is the Best Grain for Mushroom Spawn? (2)

The process of cultivating mushrooms has several distinct stages. This is because home-grown fungi are very prone to contamination in the early stages of their life cycle. Therefore, it is essential to establish a healthy mycelial network before adding it to the bulk substrate.

This is why grain spawn plays a crucial role. It provides a sterile, nutrient-rich environment for the mycelium to develop with a minimal risk of contamination. Once the grains are fully colonized, the mycelium is better able to defend itself against pathogens like bacteria and mold. Therefore, can be added to the bulk substrate relatively safely.

There are many different grains that can be used to create mushroom spawn, but some are more suitable than others. Below, we will compare the options and explore which is best. But first, let’s take a brief look at the process of creating mushroom grain spawn.

Growing Mushrooms Using Grain Spawn

Below, we have summarized how to grow mushrooms using grain spawn. However, it is not a comprehensive guide, and we recommend that you thoroughly research each step before proceeding.

Washing and Soaking the Grains

The first step involves washing and soaking the grains to rehydrate them. How long this takes will depend on their size. For example, large grains like popcorn require more soaking than smaller grains like millet, with medium-sized grains like rye being somewhere in between.

Sterilizing the Grains

The grains must be thoroughly sterilized before adding the mushroom culture. Most people do this using jars or mushroom grow bags inside a pressure cooker. The pressure cooker should be set at around 15 PSI (250°F/121°C), which is hot enough to destroy the relevant pathogens.

A simpler option is to buy pre-sterilized grains from a mycology supplier or use microwavable rice bags. While many experts agree that rice is not the best grain for growing mushrooms, this quick and easy method eliminates the need for a pressure cooker and is an easy first step into the world of mushroom cultivation.

If you use pre-sterilized grains, you can skip the soaking and sterilization steps detailed above.

Inoculating the Grains

The next stage is to inoculate the grains with your mycelium. This might take the form of a liquid culture or clean, colonized agar. Adding mushroom spores directly to grain is not recommended as it increases the risk of contamination.

Good hygiene is critical to avoid introducing mold or bacteria to the grains at this stage. This means creating a sterile environment using a still air box or a laminar flow hood.

The latter option is a relatively expensive piece of kit but could be a worthwhile investment for anyone who is serious about cultivating mushrooms. It is also best practice to wear disposable gloves and a mask when handling your uncolonized grains and mycelium.

Incubating the Grains

Once the mushroom culture or agar has been added to the sterile grains, you must wait for the mycelium to colonize them. This period is known as incubation. Depending on the variety of mushrooms you are growing, the grains may need to be incubated at a specific temperature.

As the grains colonize, they will start to turn white. This is a sign that the mycelium is developing as it should. Be vigilant for signs of contamination, such as green, black, orange, or pink patches. If these appear in your grains, dispose of them immediately.

Once the grains turn completely white, they are fully colonized, and your spawn is ready to add to the bulk substrate. Colonization can take several weeks, and some experts recommend waiting an additional five days after the grains turn white to ensure they are fully colonized.

Inoculating the Bulk Substrate

Pasteurize the bulk substrate and add the spawn, breaking it up into individual grains to maximize the surface area. Mix the spawn into the substrate evenly, paying close attention to hygiene and potential sources of contamination. Cover with a lid to retain moisture and maintain optimal conditions for colonization

Finally, wait for the bulk substrate to be fully colonized before initiating fruiting conditions. The time this takes will vary depending on factors such as the mushroom species, the substrate, the temperature, and the container size.

Initiating Fruiting Conditions

Initiate fruiting conditions by introducing fresh air to the colonized bulk substrate and mist several times a day to maintain moisture levels. Within a few days, you should see your first mushrooms starting to form!

The Best Grain for Mushroom Spawn

Now that we have explained how to grow mushrooms using grain spawn, let’s investigate the best options.

What Is the Best Grain for Mushroom Spawn? (3)

Rye

Many people consider rye to be the best grain for mushroom spawn.

It is nutritionally dense, retains moisture, and has relatively small grains with a large surface area. This is advantageous because it means more inoculation points for the mycelium and, therefore, faster colonization.

The major downside of rye is its availability. It is less common in stores than other grains, and although it can be purchased online, it may be costly.

Rye can also dry out relatively fast and should be soaked overnight for the best results.

Wheat

Wheat (sometimes known as wheat berries or wheat seeds) is another popular option.

It is similar to rye in terms of its nutritional content and surface area. It is also more widely available in many areas, making it more affordable.

The main disadvantage of wheat is that it holds less water than rye and can, therefore, dry out even more quickly.

Millet

The main advantage of using millet is that the grains are tiny. Therefore, it has a far larger overall surface area than rye or wheat, making it faster to colonize. It is also relatively resistant to contamination.

However, millet’s tiny grains can be tricky to handle, and you should be prepared for them to bounce around and get into every nook and cranny of your workspace. They can also become mushy more quickly than other grains.

Popcorn

Popcorn is widely available and is a good option for beginners. It is relatively easy to rehydrate correctly and less prone to contamination than some other grains.

The main disadvantage is that popcorn is a much larger grain than the others we have discussed so far, meaning it has a smaller surface area and is slower to colonize. It is also one of the most expensive options on our list.

Brown Rice

As we mentioned earlier, rice is not considered the best grain for mushroom spawn. It tends to get mushy quickly and is quite prone to contamination.

However, it is very affordable and widely available. Microwavable rice also comes in pre-sterilized bags, meaning you do not need a pressure cooker for this method. If you decide to go down this route, be sure to choose whole-grain brown rice rather than white rice, which can become extremely sticky.

Wild Bird Seed

Wild bird seed contains a mixture of different grains, and it is cheap and widely available. However, it has several significant downsides for mushroom cultivation.

Because wild bird seed has various grains of different sizes, it is challenging to rehydrate evenly. Furthermore, if you use wild bird seed to create grain spawn, you should avoid brands that include sunflower seed shells or cracked corn, as these ingredients can cause issues with colonization.

Other Grains

Many other grains, including barley, oats, and sorghum, can be used to grow mushrooms. In fact, you can grow mushrooms using almost any grain you have available. While some are more suitable than others, most work well enough for small-scale operations.

The Best Grain for Mushroom Spawn: Final Thoughts

Creating grain spawn is an essential step for growing mushrooms at home. It involves colonizing a cereal grain with mycelium before adding it to the bulk substrate where it will fruit.

You can use a wide variety of grains, and each of them has pros and cons. Therefore, many experts agree that the best grain for mushroom spawn is whatever you have available. For example, while many consider rye the number one choice, it can be more difficult to find than millet, popcorn, or brown rice.

The variety of mushrooms you are growing, and environmental conditions can also have a significant impact. At the end of the day, every grow is different, and finding the best grain can often be a case of trial and error.

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Published on: March 27, 2024

What Is the Best Grain for Mushroom Spawn? (2024)

FAQs

What Is the Best Grain for Mushroom Spawn? ›

The Power of Millet

What is the best ratio for grain spawn to substrate? ›

The more spawn used the faster the bulk substrate will colonize. Recommended spawn ratios are usually no less than 20% (1 part spawn to 4 parts substrate).

What are the best conditions for mushroom spawn? ›

Substrate: They aren't picky, but they do best in straw or hardwood sawdust. Humidity: High humidity levels are essential, around 80-90%. Temperature: A temperature range of 55-75°F (13-24°C) promotes healthy growth. Light: They require low levels of indirect light to trigger fruiting.

What are the best conditions for grain spawn? ›

This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions. Ideal conditions for this phase are in a dark place and temperature between 72 F – 80 F.

How long to soak grain for mushroom spawn? ›

The first step to making grain spawn is to soak some cereal grain. Soak the grain by simply placing it in a bucket or bowl, covering in water, and leaving it for 12 -24 hours. If you leave the grain for too long, it will eventually sprout- so try not to soak it for more than 24 hours or so.

What is the best grain for mycelium growth? ›

Rye Grain. If you have access to Rye, it will be your best choice most of the time. It's excellent at holding water (can hold more moisture than any other grain), and doesn't often burst or get mushy when processed. Also, mushrooms of all types seem to absolutely love rye as a source of nutrition.

What is the substrate to spawn ratio for cubensis? ›

It's important to maintain a spawn to substrate ratio of 1:3 or 1:4 for cubensis and b plus mushrooms and ensure proper sterilization or pasteurization of the mushroom substrate before inoculation to prevent contamination.

How do you encourage mushroom fruiting? ›

To initiate fruiting, lowering temperatures and increasing oxygen levels generally encourages mushroom formation. Initiation can be in the form of cold shocking, water shocking or introducing more oxygen by cutting a hole in the bag.

What speeds up mushroom growth? ›

Gypsum is a mineral that helps speed up the mushroom growing process in small amounts.

What temperature kills the mushroom spawn? ›

Mushroom cultivars differ in their response to spawn growth temperatures. However, as a general rule, most cultivars have an optimal growth temperature range of 23° to 26°C. Temperatures higher than 27°C may inhibit mycelial growth and cause injury and/or damage to the fruiting capacity of hyphae.

What grain is used for mushroom spawn? ›

Grain Spawn is mushroom mycelium grown into certified organic rye or millet grain which is ideal for inoculating sterilized or pasteurized substrates. Grain spawn is also the industry standard for high volume commercial production.

How to speed up mushroom colonization? ›

Keeping your bag at the higher end of the temperature range will encourage faster colonization. 2. After about 4-8 weeks, you should start to see mycelium growing on the substrate. You'll notice the white mycelium growing outward from the point of injection.

What makes mycelium grow faster? ›

Light: While mycelium doesn't require light to grow, a small amount can help stimulate growth. 5. Nutrition: Using a nutrient-rich substrate can also enhance growth. Remember, it's important to maintain sterile conditions to prevent contamination.

What temperature does cubensis grain spawn at? ›

cubensis colonizes most rapidly between 75-80°F (24-27°C). Temperatures higher than this range may kill the mycelium and encourage growth of contaminants, and temperatures lower than this range may slow down colonization.

What is the ratio of grain to substrate in a Monotub? ›

We frequently get asked the question, “how much grains and substrate should I purchase to spawn a gourmet monotub?” The answer is a proper ratio in your vessel of choosing. We feel the best ratio is 1:2 spawn to sub.

How much spawn for 5 lbs of substrate? ›

We recommended 1 lb of colonized grain spawn for every 5 lbs of manure or hardwood sterile substrate. We also recommend using 2.5-5ccs per 3 lb injection port bag.

How do you add grain spawn to substrate? ›

Mix in liberal amounts of spawn. Mix in well, and try not to leave large amounts of spawn on the top layer, as it will dry out easier. Cover in a thin layer of extra substrate. Soak the spawn/substrate mixture well.

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